The Northwestern University Transgenic and Targeted Mutagenesis Laboratory (TTML) is a shared resource designed to produce genetically engineered mice for research projects of investigators on the Evanston campus, in the Feinberg School of Medicine, the Robert H. Lurie Comprehensive Cancer Center, and the Stanley Manne Children’s Research Institute.
Our laboratory provides a broad, comprehensive range of services to NU investigators, including generation of transgenic mice, gene targeting of embryonic stem (ES) cells, ES cell microinjection into blastocysts to create germline competent chimera, cryopreservation and long-term storage of mouse sperm/embryos, recovery of mouse lines from cryopreserved germplasm, in vitro fertilization (for mouse line rescue), and rederivation of pathogen free mouse strains.
- TTML Faculty Advisory Committee
Meet our experienced advisors who work with us to ensure quality and efficiency in our operations
- Our Track Record / What's New
Learn about our successes in the services we provide and what's new at the laboratory
- Contact Us
Connect with lab staff
- CRISPR/Cas9 Gene Editing
Discover how we are using CRISPR/Cas9 to create targeted mutations directly in embryos or in ES cells.
- Transgenics Projects
Learn about the TTML process for producing transgenic mice
- Gene Targeting
Get details about our work in gene targeting with chimeric mice
- ES Cell Injection
Understand our process for culturing and/or injecting ES cells for investigators
Find details and pricing related to the TTML's rederivation process
- Cryopreservation of Mouse Lines
Learn more about our offerings in this critical service related to modern mouse based genomics
- Cryorecovery of Mouse Lines
Discover the processes and costs related to our cryorecovery services
Our Track Record / What's New
Since 2008, the laboratory has completed fifty gene targeting projects with a success rate of 98%.
Find out more about our track record and what's new at TTML.
TTML is sponsored by
The Center for Genetic Medicine
The Robert H. Lurie Comprehensive Cancer Center
Feinberg School of Medicine, Office of the Dean
Office of the Vice President for Research