Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

Cryorecovery of Mouse Lines

Our cryorecovery services allow the worldwide import and recovery of mouse lines from frozen embryos or sperm. Cryopreservation of mouse germplasm has become one of the most common methods for maintaining huge repertoires of genetically altered mice globally. Many animal vendors such as Jackson Laboratories, Taconic, and Charles River have begun maintaining lines as frozen embryo and/or sperm stocks. Mouse lines may also now be available through one of the following international repositories: Mutant Mouse Regional Resource Centers (MMRRC), International Knockout Mouse Consortium (IKMC),  KOMP2, or RIKEN Bioresource Center, Experimental Animal Division. The majority of the lines from these repositories are maintained as cryopreserved stocks. Cryopreservation of embryos/sperm has gained popularity in academic institutions as well, as this technology has become mainstream.

Shipment of cryopreserved material is a safe way to transport mouse lines worldwide and eliminates the need to send live animals. Cryorecovery is also an acceptable method to rederive infected mouse lines.

TTML has the capability of both shipping and receiving cryopreserved embryos and sperm. Frozen material received from either outside the institution or maintained by TTML can be thawed and surgically transferred into recipient females at any time to recover the line.