Welcome to NUSeq!
NUSeq is a new core facility that provides full next-gen sequencing (NGS) capability to Northwestern University. The core brings together in-house NGS data generation and bioinformatics support in a single integrated operation. As the only Northwestern core facility dedicated to NGS, NUSeq serves all NU researchers on both the Chicago and Evanston campuses, as well as those at external academic and commercial organizations.
Click here for a list of NGS and other genome research services we provide.
Email NUSeq@northwestern.edu, or call us at 312-503-3331 (director) or 312-503-3702 (lab).
NUSeq/Illumina Pilot Program
To support the next round of NIH grant proposals, Illumina will provide a limited quantity of sequencing or genotyping reagents at no cost to the Northwestern University NUSeq Core Facility. NUSeq, in turn, will offer pilot awards that include free reagents and reduced user fees to help NU investigators generate preliminary data.
This pilot program encourages projects to take advantage of the recently installed Illumina HiSeq 4000 and MiSeq sequencers. For proposals using the HiSeq 4000, we expect to offer up to one lane's worth of data (300-400 million reads). Depending on the scope of the NIH proposal, use of more than one lane may also be supported.
To apply for this pilot program, please submit a 500 word abstract along with an NIH-style biosketch for the Principal Investigator to Ashley Limon. The deadline for abstract submission is 11/27/17. The abstract should indicate how the sequencing will facilitate your NIH grant application. Please include the title and type (e.g. R01) of the grant application and the type of award (New, Competing Renewal, or Resubmission). Please also include the planned date for submission of your full grant proposal to the NIH.
Feedback and award notices will be provided by 12/15/17. Along with the award notice, NUSeq will send a quote for the reduced user fees associated with each project and the estimated date of results delivery, based on the amount of staff work needed for each project. NUSeq will make every effort to deliver results in a timely fashion to facilitate full grant proposal submission, but please be aware that this is not guaranteed.
New Service Offerings
High-throughput Single-cell RNA Sequencing
The 10x Genomics Chromium System for Single-Cell RNA-seq is now available at NUSeq! Large numbers of single cells (100-80,000+) can be analyzed by RNA-seq for applications like cell population heterogeneity analysis and rare cell type detection. This RNA-seq service is offered through a collaboration with the Division of Pulmonary and Critical Care Medicine. In addition to high-throughput scRNA sequencing, NUSeq also provides a manual scRNA-seq service for small numbers of single cells (<100).
Cell Line Authentication Service
NUSeq has recently developed a new service for cell line authentication in response to the increasing scientific community requirement for scientific rigor and reproducibility. This new service is based on the use of STR (Short Tandem Repeat) profiling. NUSeq uses a forensic-grade kit (AmpFLSTR® Identifiler® Plus PCR Amplification Kit) for profiling 15 tetranucleotide repeat loci and the Amelogenin marker (for gender determination). The STR marker status on the result report is then used to search existing cell line STR databases (such as that maintained by ATCC) for match.
Expanding Research Infrastructure
We are pleased to announce the installation and full operation of new Illumina MiSeq (available since September ’17) and Illumina HiSeq 4000 (since June ’17) next-gen sequencers at the NUSeq Core Facility.
The system can generate 25 million single reads or 50 M paired-end reads, up to 15 Gbp sequence data in 4-56 hours, up to 300 bp in read length. The Illumina MiSeq sequencer is used for applications including:
- Microbiome 16S rRNA sequencing
- Small genome de novo assembly and variant analysis
- Small RNA sequencing
- Targeted DNA or RNA sequencing
- Screening of CRISPR/Cas9-induced gene editing events
Illumina HiSeq 4000
The Illumina HiSeq 4000 was funded by a Searle Leadership Fund award. This sequencing powerhouse provides unparalleled data output and sample throughput with exceptional data quality. The sequencer generates 10 billion reads and 1500 Gigabases in each run, supporting a broad range of research projects including: whole genome sequencing, RNA-seq, ChIP-seq, DNA methyl-seq, microbiome shotgun sequencing, and cancer risk gene panels.
NUSeq offers extremely competitive pricing for sequencing performed using this new instrumentation. On the HiSeq 4000 – starting from $725 per lane for 300-400 million 50 bp single reads – all sequencing runs have the lowest running costs in the Chicago region and are among the lowest in the nation.
Advancing Discovery Through Cutting-Edge Genomic Technologies
Q&A with Xinkun Wang, PhD, director of the NUSeq Core Facility in the August 2017 Feinberg School of Medicine Research Office Breakthroughs newsletter.
NUSeq featured in the Northwestern Core Facilities video
Xinkun Wang, PhD
Research Associate Professor
300 E. Superior Street
Telephone: (312) 503-3331
Fax: (312) 503-3210
Matthew Schipma, PhD
Research Assistant Professor
750 N. Lake Shore Drive
Telephone: (312) 503-5324