Gene Targeting Services and Rates

Contact us for more information on rates. For information on what fees include, see below.

Initiation Fee

A non-refundable fee covers assessment of DNA, provision of reagents and electroporation setup.

Phase I: ES Cell Targeting

• DNA quantification and gel electrophoresis analysis
• The targeting construct is electroporated, ES cells are placed under drug selection and 200-250 drug-resistant colonies are picked
• Clones are provided to the investigator for DNA isolation and genotyping
• If no targeted clones are identified, a second electroporation will be performed and additional clones (up to a total of 480) will be provided for this same fee
• Projects are limited to two electroporations per construct

Phase I Guarantees

• Based upon our continued success and a continual evaluation of ES cell stocks, we guarantee the efficiency of targeting of the ES cells and their germline transmission.
• Homologous recombination (targeting) is locus dependent. Therefore, we cannot guarantee that targeting will occur with a particular construct.
• Germline is not guaranteed for clones stored in 96 well plates for longer than two months.

Phase II: Production of Chimeric Mice

• Thaw, expansion and long-term storage of 1-5 ES cell clones
• Blastocyst microinjection of one to four targeted ES cell clones: initially, one to two clones are injected into approximately 24 blastocysts per clone. This is generally a sufficient number to generate at least five highly chimeric males per clone. If chimerism is low to moderate, another one or two targeted clones are injected.

Phase II Guarantees

• Generation of three to four highly chimeric mice (>50 percent chimerism) from any of one to three targeted clones generated by the TTML using our standard laboratory ES cell stocks
• We cannot guarantee germline transmission of any one particular clone, however, germline transmission of the targeted ES cells is guaranteed
• Germline transmission is not guaranteed for other TTML ES cell stocks, including PRX-B6 and PRX-B6T, however, we guarantee that the parental lines are germline competent at the passage used for electroporation

Costs include the purchase of all mice used for the project, the housing of the surrogate females and the housing of pups for up to one week post-weaning.

Additional Fees

• Additional clones can be expanded for a fee
• Housing costs: investigators will be charged for daily animal care if transfer of animals exceeds one week post weaning
• Use of other, non-routine strains for microinjection; includes the purchase and per diems of singly housed males for length of project