Cryorecovery of Mouse Lines Services and Rates

Embryo Cryorecovery

Embryos frozen and frozen/maintain in TTML dewars can be recovered at the request of the investigator at any time.

Embryos received from outside institutions will be thawed according to the protocol provided by the supplier; recovery of viable embryos cannot be guaranteed.

Services include:

Coordination of embryo shipment
Thaw, assessment and surgical transfer of viable embryos into recipient females
Ear tag and tissue biopsy (if necessary) of weanling pups
Bleeding of recipient females; pathogen testing
Transfer of SPF pups to your protocol

Costs include embryo thaw and surgery, purchase of wild-type mice used for the project, housing of the surrogate females, and the housing of pups for up to one week post-weaning.

Investigators are responsible for the following costs:

Shipping costs: all purchase and shipping costs required to obtain embryos
Housing costs: per diems for weaned pups if mice have not been transferred within one week of wean date
Pathogen testing: all costs associated with any additional required testing
Transport costs: all transport costs associated with the transfer of animals to different vivaria

Sperm Cryorecovery

Sperm frozen and stored by the TTML can be recovered at the request of the investigator at any time.

Sperm received from outside institutions will be thawed according to the protocol provided by the supplier; recovery of viable embryos cannot be guaranteed.

Services include:

Help coordinating shipment of the cryopreserved sperm from outside sources
purchase of wild-type females for IVF
IVF using thawed sperm and wild-type oocytes
Surgical transfer of IVF-generated zygotes directly into recipient females
Ear tag and tissue biopsy (if necessary) of weanling pups
Bleeding of recipient females, if necessary, for pathogen testing
Transfer of SPF pups to your protocol

Costs include sperm thaw and surgery, purchase of wild-type mice used for the project, housing of the surrogate females, and the housing of pups for up to one week post-weaning.

Investigators are responsible for the following costs:

Shipping costs: all purchase and shipping costs required to obtain cryopreserved sperm
Housing costs: per diems for weaned pups if mice have not been transferred within one week of wean date
Pathogen testing: all costs associated with any additional required testing
Transport costs: all transport costs associated with the transfer of animals to different vivaria