Generation of plasmid-based and BAC DNA transgenic mice in a B6SJLF2, FVB or C57BL/6 background.
Final purification of plasmid-based transgenes or analysis of BAC DNA transgenes
Pronuclear injection of enough embryos to produce 20-25 pups
Wean and ear tag 2 week old pups; take tail biopsy for genotyping
Transfer of founders to investigator
Typically, transgenesis rates of 10-25% are achieved resulting in the generation of 2-7 transgenic founders, although results can vary significantly among constructs.
If no founders are identified from the initial set of injections, a consultation will be requested. If the genotyping assay is determined to be accurate and adequate, a second set of injections will be performed.
At least two transgenic founders or a maximum of 400 injected embryos are guaranteed.
Generation of founders using special strains is not guaranteed
Up to 400 embryos/construct will be injected with no guarantee of founders for projects in which the investigator opts to proceed with injections using suboptimal DNA
Transgenic expression is not guaranteed
Purchase of all animals used for the project
Housing of the surrogate females
Housing of pups for up to 1 week following weaning
Housing costs: daily animal care if genotyping exceeds more than 1 week after tails snips are provided
Use of other, non-routine strains for microinjection; includes the purchase and per diems of singly housed males for length of project