Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

Production of Chimeric Mice

Potentially targeted clones are thawed from the 96 well plates, expanded, and frozen for stable long-term storage in LN2. Expanded clones confirmed as correctly targeted by the investigator are scheduled for blastocyst microinjection. Targeted ES clones are microinjected into blastocysts to generate chimeric mice.

Expansion and confirmation of targeted clones

Blastocyst injections

Timeline

Investigator responsibilities:

Frequently Asked Questions

Costs and Services

See Gene Targeting costs and services