Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

ES Cell Targeting

Targeting vector DNA is electroporated into ES cells, the electroporated cells are then grown in drug selection media, and surviving colonies are picked into 96 well plates. Colonies growing in the 96-well plates are divided into replica plates: one set is frozen and stored at -80°C for future use, the second set is provided to the investigator for DNA isolation and genotyping.

Generation of targeted clones

Gene targeting timeline

Investigator responsibilities for gene targeting projects:

Frequently Asked Questions

Costs and Services

See Gene Targeting costs and services