The Transgenic and Targeted Mutagenesis Laboratory's goal has been to provide a minimum of two (our current average is 5.8) transgenic founders per construct and a minimum of three (our current average is ten) highly chimeric males for each gene targeting project to achieve germline transmission. Since relocating to the Chicago campus, the laboratory has completed fifty gene targeting projects with a success rate of 98%: only one project has not resulted in germline transmission.
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Overall, 98% (49 of 50) of our gene targeting projects have resulted in germline transmission of the targeted allele.
Germline transmission rates for ES cell clones received from other academic institutions or international consortiums.
The Transgenic and Targeted Mutagenesis Laboratory (TTML) has successfully frozen over 684 mouse lines since establishing embryo cryopreservation in May 2007 and sperm cryopreservation in October 2008.
The Transgenic and Targeted Mutagenesis Facility has successfully recovered 90% (36 out of 40) of imported cryopreserved lines, and 100% (14 from embryos, 12 from sperm) of lines cryopreserved and stored in our laboratory.