Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

Human Cell Line Authentication through STR Profiling

"An authentic cell line is a cell line of a specific species that retains its unique functionality and characteristics. Cell line authentication is a set of tests intended to demonstrate that a cell line is what it is reported to be. Short Tandem Repeat (STR) Profiling is a form of interspecies cell line authentication that looks for the variation of tandem repetitive sequences in your cell line. STR profiling is rapid, reproducible, and uses a standardized PCR-based method for the authentication of human cell lines" –From ATCC