Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

RNA Sequencing

RNA sequencing, or RNA-seq, is the latest technology to study the transcriptome, i.e., the full set of RNA transcripts as genome readouts in a cell or population of cells. This technology directly sequences RNA molecules in the transcriptome, and compared to microarray, it does not make assumptions as to which parts of the genome are transcribed. After sequencing, the generated reads are usually mapped to the reference genome to identify their genomic origin. The total number of reads mapped to a particular genomic region represents the level of transcriptional activity in the region. The more transcriptionally active a genomic region is, the more copies of RNA transcripts it produces, and the more RNA-seq reads it generates. Essentially RNA-seq analysis is a counting game.

NUSeq provides six types of RNA-seq services as detailed below.

To get started, core users may refer to core documents: RNA-seq Workflow Steps and Examples and the RNA-seq Decision Tree to help determine the type of RNA-seq service needed.

The FAQ section of this website contains more information on various aspects of carrying out a successful RNA-seq experiment.

RNA-seq Library Prep Pricing

Library construction costs for the different types of RNA-seq are listed on the Core Pricing page.

RNA Sequencing

Frequently Asked Questions