The methylation of cytosines is a major epigenomic mechanism that modulates the primary genomic code. This leads to the formation of 5-methylcytosines (5mCs) at select sites of the genome. Cytosine methylation regulates gene expression and chromatin remodeling, and as a result plays important roles in many biological functions including embryonic development, cell differentiation, and stem cell pluripotency. Abnormal DNA methylation can lead to diseases, such as cancer.
While DNA methylome analysis has for many years been conducted with the use of microarrays (such as the Illumina Infinium MethlyationEPIC BeadChips), DNA methylation sequencing is a newer genomics technology that maps cytosine methylation in the genome. To provide a representative picture of DNA methylation across the genome, NUSeq uses RRBS, or Reduced Representation of Bisulfite Sequencing, an approach that strikes a balance between genome coverage and cost-effectiveness/data manageability.
To perform RRBS, NUSeq starts with high-quality genomic DNA. Firstly, the genomic DNA is digested with a methylation-insensitive restriction enzyme, such as MspI. The digested DNA fragments are then subjected to major steps, including adapter ligation, bisulfite conversion, and PCR, to generate a library for sequencing.
|RRBS DNA Methylation Sequencing Library Prep Pricing|