Microarray is the first high-throughput genome technology developed in the mid-1990s. Over the years, this technology has seen tremendous growth and maturation. It was orginally developed for gene expression profiling, but its application soon diversified into other areas, including genotyping, DNA methylation analysis, and copy number variation analysis with comparative genomic hybridization (CGH). Attested by the large amounts of data deposited in major genomics databases such as Gene Expression Omnibus (GEO), microarray technology has significantly advanced high-throughput genome research for over two decades.
Different from next-gen sequencing, microarray is based on hybridization of nucleic acid molecules present in research samples to pre-designed probes. Signal readouts from the hybridization are then used to analyze gene expression pattern, DNA sequence polymorphism, epigenetic modification, genome instability, etc. Based on how probes are generated and immobilized, there are different microarray platforms. The NUSeq Core provides support to two of the most widely adopted microarray platforms, i.e., the Affymetrix GeneChip and the Illumina BeadArray systems. NUSeq also provides bioinformatics services to data generated on both platforms.