Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

NUSeq Announces the Illumina HiSeq 4000, Significantly Expands Sequencing Capacity at Northwestern

The Center for Genetic Medicine and Northwestern Feinberg School of Medicine Research Core Facilities announce the installation and full operation of a new Illumina HiSeq 4000 Next-Gen Sequencer at the NUSeq Core Facility. The arrival of this sequencer marks a major expansion of next-gen sequencing capabilities at Northwestern after the establishment of NUSeq in 2015. The acquisition of this instrument was provided by the Searle Leadership funds.

Available to all Northwestern researchers, the Illumina HiSeq 4000 provides unparalleled data output and sample throughput with exceptional data quality. This sequencer can generate 10 billion reads and 1500 Gigabases in each run supporting a broad range of research projects including whole genome sequencing, RNA-seq, ChIP-seq, DNA methyl-seq, microbiome shotgun sequencing, and cancer risk gene panels.

NUSeq offers extremely competitive pricing for sequencing performed using this new instrumentation. Starting from $725 per lane for 300-400 million 50 bp single reads, all sequencing runs have the lowest running costs in the Chicago region and are among the lowest in the nation. Information on sequencing services and pricing on the HiSeq 4000 is available on the Core’s website. Services can be requested through NUcore. Initial project consultation is encouraged and provided free by contacting the Core.

With the addition of this HiSeq 4000 and the more recently purchased Illumina MiSeq, NUSeq has a fleet of next-gen sequencers designed to meet the needs of Northwestern’s researchers. NUSeq is now providing state-of-the-art next-gen sequencing capabilities coupled with integrated bioinformatics analysis to support Northwestern’s biomedical research.